维甲酸对脑出血大鼠LINGO-1表达的影响

摘要

Objective To explore the effect of retinoic acid on expressions of LINGO-1 in rats with intracerebral hemorrhage (ICH).Methods 72 SD rats were randomly divided into sham operation group,model group,and retinoic acid group.Observation point was made at 1,3,7,14d after ICH.Degree of Neurological functional defects was evaluated by Longa's score.RT-PCR and Western blot were applied to detect the changes in the expressions of LINGO-1 mRNA and protein.Results Longa's score and LINGO-1 protein were decreased at 14d after ICH in retinoic acid group compared with those in model group (P ＜ 0.05).The LINGO-1 mRNA was decreased at 7d and 14d after ICH in retinoic acid group compared with model group (P ＜ 0.05).Conclusions The expressions of LINGO-1 after ICH are increased significantly.It up-regulates at early stage,then down-regulates.Retinoic acid can decrease LINGO-1 mRNA,protein expressions and neurological score.%目的 观察脑出血后LINGO-1表达的变化,探讨维甲酸对脑出血后LINGO-1表达的影响.方法 将72只SD大鼠随机分为假手术组、模型组和维甲酸治疗组,选取造模后1d、3d、7d和14d为观察点.制备脑出血模型,Longa评分法评价神经功能缺损程度,RT-PCR法检测LINGO-1 mRNA的表达,Western blot法检测LINGO-1蛋白的表达.结果 模型组Longa评分7d最高,14d出现下降;LONG-1 mRNA 3d表达最高,7～ 14d出现下降;LONG-1蛋白7d到高峰,14d出现下降.维甲酸治疗组在14d Longa评分较模型组下降(P＜0.05);在7d和14d HNGO-1mRNA表达较模型组下降(P＜0.05);在14d LINGO-1蛋白表达较模型组下降(P＜0.05).结论 脑出血后LINGO-1表达明显上调,呈先上升后下降的变化规律.维甲酸可以降低LINGO-1 mRNA和蛋白的表达及神经功能评分.