Impact of Triple Combinations of Retinoic Acid, Mold Spores and Citral on the F344 Rat Lung Tissue Pathology

摘要

The impact of retinoic acid (All Trans Retinoic Acid; ATRA) and Mold spores (MLD) in the development of lung pathologyand in vivo tissue remodeling have not been well established in the literature. In addition, the role of citral (inhibitor ofretinoid function) in the improvement of lung pathology has not been ascertained in animal studies. Therefore, it ishypothesized that ATRA and Mold (MLD) exposure will sensitize lung tissues leading to lung tissue pathology and thatCitrals (C1 and C2) will reverse, ameliorate or improve the associated pathological damage to lung tissues. The study used anIACUC approved between-subject in vivo randomized split plot factorial design (F344 rat model; N=40). Animals wereexposed to seven different treatments including untreated control, MLD, ATRA, Citrals (C1 and C2) and their MLDcombinations (MLD+ ATRA+ C1, and MLD+ ATRA+ C2) by intra-peritoneal route. Rat weight and blood data werecollected on Days 1 and 21, all animals were sacrificed on day 21, and lung tissues were processed for histopathology.Results from weight and blood data (ANOVA and Duncan) as well as from histopathological analyses supported the findingsthat exposure of F344 rats to MLD combinations with ATRA and Citrals showed various levels of lung tissue damage thatwere impacted by either C1 or C2 exposure. This promising study showed impressive responses on the interaction of MLD,Citrals, and ATRA as related to their impact on associated lung tissue pathologies.