Objective To establish a pore column RP-HPLC method for determination of emodin and chrysophanol in dantong capsule. Methods A ODS inverse pore column( 2. 1 mm×250 mm,5 μm )was used. The mobile phase:acetonitrile —0. 1% phosphoric acid( v/v,85:15 ),the flow rate was 0. 4 mT,/min,the detection wavelength was 254 nm,the column temperature was 30 t. Results The linear range of chrysophanol and emodin was 0. 01 ~0. 15 μg/g( r =0. 999 9 )and 0. 02 ~0. 24 μg/g( r =0. 999 7 ). Conclusion This method has low cost,high sensitivity,good reproducibility, and it can be used as a standard method for determination of emodin and chrysophanol in dantong capsule.%目的 建立反相高效液相细孔柱法,测定复方胆通胶囊中大黄素和大黄酚含量.方法 色谱柱为ODS反相细孔柱(2.1 mm×250 mm,5μm),流动相为乙腈:0.1%磷酸(v/v)=85:15,流速为0.4 mL/min,检测波长为254 nm,柱温为30 ℃.结果 复方胆通胶囊中大黄酚含量测定在0.01~0.15 μg/g之间具有很好的线性关系(r=0.999 9),大黄素含量测定在0.02~0.24 μg/g之间具有很好的线性关系(r=0.999 7).结论 实验所建立反相高效液相细孔柱法测定复方胆通胶囊中大黄酚和大黄素含量的方法成本低、灵敏度高、重现性好,可作为检测胆通胶囊中大黄酚和大黄素含量的标准方法.
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