CIK 联合 DDP对卵巢癌细胞株 SKOV-3的杀伤效应

摘要

Objective To investigate the anti-tumor effects of cytokine-induced kill cells ( CIK) combined with chemo-therapeutic drug DDP against ovarian cancer cell lines SKOV-3.Methods Peripheral blood mononuc1ear ce1ls ( PBMC) of healthy people were stimulated by different cytokines ,and were induced into CIK cells .CIK cells were cultured for 14 days as ef-fector cells.The phenotype of CIK cells were analyzed by flow cytometer .SKOV-3 cells were cultured with the CIK cells culturing supernatant for 24 and 48 hours and the SKOV-3 cells apoptosis was analyzed by flow cytometer .Killing experiment were divided into A1~4 CIK group;B1~7 DDP group and C1~2 CIK combined with DDP group .The antitumor effects were measured by MTT .Re-sults The CD3 +CD56 +double positive cel1 was up to 38.7 percents after 14 days’cultivation.The apoptosis rates of SKOV-3 cell lines were (12.30 ±1.47) %and (27.13 ±2.03) %respectively after 24 hours and 48 hours of coculture with CIK cells supernatant .The killing effect of CIK and DDP increased with the increase of effector-target ratio and drug concentration .The kill-ing effect of combination of CIK and DDP was obviously higher than that of each single factor .Conclusion CIK cells have strong anti-tumor effect against SKOV-3 cells by inducing apoptosis of SKOV-3 cells,and it can enhance the anti-tumor effects of DDP a-gainst SKOV-3 cells when CIK and DDP are combined together .%目的：探讨多种细胞因子诱导的杀伤细胞（ CIK）联合DDP对卵巢癌细胞株SKOV-3的杀伤效应。方法用健康人外周血单核细胞（ PBMC）在体外用多种细胞因子诱导成CIK细胞，于培养14天收获CIK细胞作为效应细胞，流式细胞术分析其表型特征。将CIK细胞培养液上清作用于SKOV-3细胞，于培养24及48 h用流式细胞仪检测SKOV-3细胞的凋亡情况。杀伤实验共分为A1～4 CIK作用组；B1～7 DDP作用组；C1～2 CIK联合DDP作用组；采用MTT法检测CIK和DDP对SKOV-3的生长抑制效应。结果 CIK 细胞体外培养14 d时CD3＋CD56＋双阳性细胞数量达38．7％；SK-OV-3与CIK细胞培养液上清共培养24 h及48 h后凋亡率分别为（12．30±1．47）％和（27．13±2．03）％；CIK及DDP对SKOV-3细胞的抑制率随着效靶比和药物浓度的提高及作用时间的延长而提高。而两者联合对SKOV-3的杀伤作用明显高于单一因素。结论 CIK细胞可通过诱导卵巢癌SKOV-3细胞凋亡发挥较强的杀伤作用。联合CIK治疗可明显增强DDP对卵巢癌SKOV-3细胞的杀伤作用。