SiRNA 沉默 TRPC1基因对肺腺癌 A549细胞增殖和侵袭的影响观察

摘要

Objective To observe the effect of silence TRPC 1 genes in SiRNA on proliferation and invasion of lung ade-nocarcinoma A549 cell.Methods TRPC1-SiRNA genes were marked with special materials , the most appropriate transfection concentration and time was identified ,and then the blank control group and transfection reagent control group were cultivated ac -cording to the most appropriate concentration and time .Through MTT tests and invasion tests ,cell proliferation and invasion ability of the experimental observation group ,the blank control group and the transfection reagent A 549 control group were observed and compared.Results The transfection of TRPC1-SiRNA for A549 cells had the highest concentration at 48 h and 100 mmol/L,so the time and concentration of the other 2 control groups were set at 48 h and 100 mmol/L for concentration .The proliferation time of the TRPC1-SiRNA gene transfection for A549 cell was significantly less than that of non-transfection group and transfection rea-gent control group,there had statistical difference (P<0.05).The septum-permeating cells of the TRPC1-SiRNA gene transfec-tion for A549 cell in the invasive experiments was significantly less than that of non-transfection group and transfection reagent control group,there had statistical differences (P<0.05).Conclusion SiRNA silence TRPC1 genes can inhibit the proliferation of A549 cells and reduce the invasive ability of A 549 cells.%目的：观察SiRNA沉默TRPC1基因对肺腺癌A549细胞增殖和侵袭的影响效果。方法 TRPC1-SiRNA基因用特殊物质标记后，找出最适宜的转染浓度和时间，按照最适宜浓度和时间，培养空白对照组和转染试剂对照组，采用MTT法检测实验组、空白对照组以及转染试剂对照组的A549细胞增殖时间，通过侵袭实验观察3组A549细胞的侵袭能力。结果 TRPC1-SiRNA基因转染A549细胞48 h和100 mmol/L浓度下转染速率最快，因此两个对照组的时间和浓度也设定为48 h和100 mmol/L。 TRPC1-SiRNA基因转染A549细胞增殖时间明显短于未转染组和转染试剂对照组，差异均具有统计学意义（P＜0．05）。 TRPC1-SiRNA基因转染A549细胞侵袭实验中穿膜细胞数实验组明显少于未转染组和转染试剂对照组，差异均具有统计学意义（ P＜0．05）。结论 SiRNA沉默TRPC1基因可抑制A549细胞的增殖，降低A549细胞的侵袭能力。