The present invention provides a kind of methods for using a position-specific score matrix method to identify siRNA target motif in a transcription. The present invention also provides a kind of method of off-target gene for using a position-specific score matrix method to identify a siRNA. The present invention further provides a kind of methods for designing siRNA higher noise suppressed effect and characteristic. The present invention also provides high noise suppressed effect and characteristic in a library of the siRNA including siRNA.
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