酪氨酸激酶 ALK、ROS1、RET 融合基因与肺癌临床特征的关系分析

摘要

Objective To explore the relation between tyrosine kinase ALK ,ROS1,RET fusion gene and clinical fea-tures of lung cancer.Methods The gene expression for ALK,ROS1,RET in 410 cases of lung cancer were tested by Western blot.The clinicopathological characteristics of patients with positive and negative ALK 、ROS1、RET gene mutation were compared. Results Gender and pathological types between patients with positive ALK 、ROS1 and RET gene mutation had significant differ -ence(P <0.05).There was no cases with 2 types of gene mutation;There had no significant difference in gender ,age,smoking history,pathological type between patients with positive and negative gene mutation (P >0.05).Conclusion ALK,ROS1,RET positive mutation gene have different clinicopathological characteristics ,the 3 gene are mutually exclusive in lung cancer .%目的：探讨肺癌酪氨酸激酶 ALK、ROS1、RET 融合基因与其临床特征的关系。方法采用蛋白免疫印迹法检测410例肺癌肿瘤组织标本中 ALK、ROS1、RET 基因蛋白的表达情况，分析 ALK、ROS1、RET 基因突变阳性患者的临床病理特征，并与阴性患者进行比较。结果ALK、ROS1基因突变阳性患者性别和病理类型与 RET 基因突变阳性患者比较有显著差异，差异具有计学意义（P ＜0．05）；所有基因突变阳性患者未发现同时存在2种及以上的基因突变同时发生的情况；基因突变阳性患者与阴性患者在性别、年龄（岁）、抽烟史、病理类型方面比较，差异无统计学意义（P ＞0．05）。结论ALK、ROS1、RET 突变阳性患者临床病理类型上有各自特征，三种基因在肺癌的表达中相互排斥。