人参皂甙 Rgl 体外抑制人骨肉瘤肿瘤干细胞增殖的研究

摘要

Objective To investigate the inhibitory role of ginsenoside Rg 1 on proliferation of human osteosarcoma canc -er stem cells in vitro.Methods MG-63 human osteosarcoma cell were cultured in vitro ,then observed under the microscope and received CD133 immunofluorescence staining.The study was divided into normal saline water group ,20 μg/mL ginsenoside Rg1 group and 40 μg/mL ginsenoside Rg1 group.Inhibitory effect of ginsenoside Rg1 on proliferation of cancer stem cells of human osteosarcoma cells in vitro was detected by MTT colorimetric method .Induction of apoptosis of ginsenoside Rg1 on proliferation of cancer stem cells of human osteosarcoma cells in vitro was measured by DAPI staining method ,optical density and area density were analysed.Results MG-63 human osteosarcoma cell cultured in vitro were CD133+ positive.MTT assay showed that inhibi-tory rates of 20 μg/mL and 40 μg/mL ginsenoside Rg1 were 23.47% and 41.84%,respectively,which were obviously lower than that of normal saline group(P <0.05,P <0.01).DAPI staining method showed that compared with normal saline water group , optical density and area density of cancer stem cells of human osteosarcoma cells in vitro remarkably decreased ,there had statisti-cal difference (P <0.01).Conclusion Ginsenoside Rg1 can inhibit the proliferation of cancer stem cells of human osteosarco -ma cells in vitro.%目的：探讨人参皂苷 Rg1体外抑制人骨肉瘤肿瘤干细胞的作用。方法体外培养 MG－63人骨肉瘤细胞，镜下观察并行 CD133免疫荧光染色；实验分为生理盐水组、20μg／mL 人参皂苷 Rg1组和40μg／mL 人参皂苷 Rg1组；采用四唑盐比色法（MTT）检测人参皂苷 Rg1对人骨肉瘤肿瘤干细胞增殖抑制作用；DAPI 核染检测人参皂苷 Rg1诱导人骨肉瘤肿瘤干细胞凋亡，并分析细胞的光密度和面密度变化。结果培养 MG－63人骨肉瘤细胞 CD133呈阳性反应；MTT 法实验结果显示：20μg／mL 和40μg／mL 人参皂苷 Rg1对人骨肉瘤肿瘤干细胞的抑制率分别为23．47％和41．84％，明显低于生理盐水组（P ＜0．05，P ＜0．01）；DAPI 核染检测结果显示：与生理盐水组比较，人参皂苷 Rg1组人骨肉瘤肿瘤干细胞的光密度和面密度值均明显减小，差异有统计学意义（P ＜0．01）。结论人参皂苷 Rg1对人骨肉瘤肿瘤干细胞的增殖具有抑制作用。