高效液相色谱法测定清心明目上清丸中盐酸小檗碱的含量

摘要

目的 建立清心明目上清丸中盐酸小檗碱的含量测定方法.方法 采用高效液相色谱法,色谱柱:AgilentZORBAX SB-C18(250mm×4.6 mm,5 μm),流动相:磷酸盐缓冲液[0.05mol/L磷酸二氢钾和0.05mol/L庚烷磺酸钠(1:1),含0.2%三乙胺,并用磷酸调节pH值至3.0]-乙腈(70:30);检测波长:345 nm;流速:1.0 ml/min;柱温:35℃.结果 盐酸小檗碱在8.112～81.12μg/ml范围内呈良好的线性关系,平均回收率为96.59%,RSD为1.6%,n=5.结论 本法可用于清心明目上清丸中盐酸小檗碱的含量测定,控制质量.%Objective To determine the content of berberine hydrochloride in Qingxin Mingmu Shangqing Pills. Methods High performance liquid chromatography analysis was carried out using Agilent ZORBAX SB-C18 (250 mm × 4.6 mm, 5 μm) and phosphate buffer-acetonitrile (73: 30 ) as the mobile phase.The buffer solution was a mixture of 0.05 mol/L solution heptanesulfonate( 1:1 ), containing 0.2％ of TEA. The phosphoric acid was adjusted to a pH of 3. 0. The detection wavelength was 345 nm, the flow rate was 1.0 ml/min, and the column temperature was 35℃. Results The linear range was 8. 112-81.12 μg/nl. The average recovery was 96.59％ and RSD was 1.6％. Conclusion The method can be used for quality control of Qingxin Mingmu Shangqing Pills.