响应面法优化黄秋葵多糖提取工艺研究

摘要

以黄秋葵为原料,采用响应面法优化黄秋葵多糖的提取工艺参数.在单因素试验的基础上,选定提取温度、提取时间和pH三个主要因素,通过3因素3水平Box-Behnken中心组合试验,建立数学模型,优化黄秋葵多糖的提取工艺.结果表明黄秋葵多糖最佳提取条件为:提取时间2.75h、提取温度90℃、pH值2.1.在此最佳条件下,黄秋葵多糖的得率为29.77%.%In this manuscript,the extraction process of polysaccharides from okra was established and optimized by response surface methodology.First,single-factor experiments were carried out.On the basis of single-factor experiments,including extraction time,extraction temperature and pH.mathematical model was established using a 3-factor,3-level Box-Behnken experimental design,to obtain the optimum extraction conditions.The results suggested that the optimal extraction conditions were extraction time of 2.75 h,extraction temperature of 90℃ and pH of 2.1.Under the above optimized conditions,the yield of polysaccharides was 29.77%.