莲花白多酚的提取及纯化工艺研究

摘要

为了获得莲花白多酚最佳提取工艺参数,采用4因素响应面试验设计进行试验,并通过静态吸附-解吸附试验对5种大孔树脂进行筛选,并确定大孔树脂纯化参数.结果表明:(1)最佳提取条件:液料比78∶1(mL·g-1),乙醇体积分数50%,超声时间40min,温度60℃.(2)筛选出AB-8树脂,最佳纯化条件为:上样浓度0.463mg·mL-1,上样量100mL,乙醇体积分数60%(pH=7),洗脱剂用量40mL.在该条件下纯化倍数接近4倍,表明AB-8大孔树脂对莲花白多酚具有较好的纯化效果.%Cabbage polyphenols were optimized using a four-factor response surface methodology (RSM).Macroporous resin was selected by static adsorption-desorption experiments from among five kinds of macroporous resins to obtain the optimum extraction parameters of cabbage polyphenols.The macroporous resin purification parameters were determined.The results showed that:(1) The optimal extraction conditions were as follows:ratio of liquid to solid was 78∶1 (mL·g-1),ethanol volume fraction was 50%,ultrasonic time was 40 min and temperature was 60 ℃.(2) The optimum purification conditions were as follows:loading concentration 0.463 mg·mL-1,sample loading volume of 100 mL,with 40 mL of 60% ethanol(pH=7) to elute.Under these conditions,the purification factor was nearly 4-fold,indicating that macroporous resin AB-8 has better purification effect for polyphenols from cabbage.