基于基因数据库及平台分析SERPINA3在胶质母细胞瘤中的表达及意义

摘要

Objective To explore the expression and clinical significance of SERPINA3 gene in glioblastoma. Meth-od The data of SERPINA3 expression in different glioblastoma cohorts were retrieved from Oncomine database, and the relationship between the expression of SERPINA3 and the survival status of glioblastoma was analyzed using these data and R2 database. Small interfering RNA targeting SEPRINA3 was designed and synthesized, and then transfected in-to U87 human glioblastoma cell line. The expression of SEPRINA3 in U87 cells was detected using western blot test. The effect of downregulation of SERPINA3 gene expression on the proliferation and invasion of U87 cells was detected by CCK-8 proliferation assay, clone forming assay and Transwell invasion assay. Result In the 4 studies indentified form Oncomine database, the expression of SEPRINA3 in globlastoma was higher than that in normal brain tissue, and the re-sults of R2 database retrieval showed that the survival status of the glioblastoma cohort with high SERPINA3 expression was significantly inferior to that of low expression cohort. Small interfering RNA transfection could successfully inhibit the expression of SERPINA3 in U87 cells, and the proliferation and invasion abilities in vitro of the cells were significant-ly decreased. Conclusion The expression of SERPINA3 is up-regulated in glioblastoma cells, which is correlated with poor prognosis. The inhibition of the expression of SERPINA3 can reduce the proliferation and invasion abilities of glio-blastoma cells, which is expected to be a potential new molecular therapeutics target.%目的 研究SERPINA3基因在胶质母细胞瘤中的表达和意义.方法 检索Oncomine数据库中不同胶质母细胞瘤队列SERPINA3的表达情况,结合R2数据库分析SERPINA3的表达与胶质母细胞瘤生存状况的关系.设计并合成靶向SERPINA3的小干扰RNA,转染U87人胶质母细胞瘤细胞系;采用Western blot方法检测U87细胞中SERPINA3的表达;通过CCK-8增殖实验、克隆形成实验和Transwell体外侵袭实验检测SERPINA3基因表达下调对U87细胞增殖和侵袭能力的影响.结果 Oncomine数据库中符合筛选条件的4项研究中胶质母细胞瘤SERPINA3相比正常脑组织呈高表达,R2数据库检索发现胶质母细胞瘤SERPINA3高表达队列生存明显劣于低表达队列.U87细胞转染小干扰RNA可成功抑制SERPINA3的表达,且其增殖和体外侵袭能力显著下降.结论 SERPINA3在胶质母细胞瘤中表达水平增高且与预后不良有关.抑制SERPINA3的表达可降低胶质母细胞瘤细胞的增殖和侵袭能力,可能成为新的分子治疗靶点.