杨梅素诱导人宫颈癌HeLa细胞凋亡及凋亡通路的研究

摘要

Objective To investigate the activity of myricetin on proliferation ,apoptosis and to study the apoptosis signaling pathway on human cervical cancer HeLa cells .Methods HeLa cells were cultured in vitro ,and MTT and SRB were performed to observe the proliferation .Flow cytometry was utilized to measure the apoptosis and analysis of the cell cycle .The expressions of caspase‐3 ,caspase‐9 and survivin were determined by Western Blot .Results Myricetin could inhibit the proliferation of HeLa cells in vitro ,in a concentration and time‐dependent manner .Myricetin could elevate the apoptosis rates ,and cell cycle was blocked in S phase .Myricetin was able to promote the expressions of caspase‐3 and caspase‐9 ,inhibit the expression of survivin .Conclusion Myricetin could inhibit the proliferation of HeLa cells ,induce apoptosis ,and the apoptosis pathway propably involves both intracel‐lular and extracellular pathways .%目的：研究杨梅素体外对人宫颈癌 HeLa细胞增殖、凋亡及凋亡通路的影响。方法体外培养人宫颈癌 HeLa细胞，M T T和SRB法检测细胞增殖抑制率；流式细胞仪检测细胞凋亡，计算机分析细胞周期；Western Blot法检测caspase‐3、caspase‐9和survivin含量。结果杨梅素对HeLa细胞的体外增殖具有抑制作用，并具有浓度和时间依耐性；杨梅素体外诱导 HeLa细胞凋亡，并将细胞周期阻滞在S期；杨梅素促进caspase‐3和caspase‐9蛋白表达，抑制survivin蛋白表达。结论杨梅素可抑制HeLa细胞的增殖，诱导其凋亡，其凋亡途径同时涉及细胞内途径和细胞外途径。