高频重复经颅磁刺激对脑缺血后海马BDNF、VEGF和Nestin表达的影响

摘要

目的：研究高频重复经颅磁刺激（rTMS）对脑缺血大鼠缺血侧海马BDNF、VEGF和Nestin表达的影响。方法：SD雄性成年大鼠16只，随机分为模型组、rTMS组、rTMS＋生理盐水（NS）组和rTMS＋H89组各4只，rTMS＋NS组和rTMS＋H89组分别侧脑室注射NS和H89，4组均建立大脑中动脉栓塞（MCAO）模型，除模型组外均给予7 d 20 Hz rTMS治疗，应用Western Blot方法检测缺血侧海马pCREB、BDNF、VEGF和Nestin表达。结果：rTMS组pCREB、BDNF、VEGF和Nestin表达较模型组均增加（＜0.05），rTMS＋H89组pCREB、BDNF、VEGF和Nestin表达较rTMS＋NS组均减少（＜0.05）。结论：高频rTMS能使脑缺血后海马区BDNF、VEGF表达增加，并进一步促进Nestin表达，PKA-CREB通路的调节可能是其机制之一。%Objective: To investigate the effects of high-frequency repetitive transcranial magnetic stimulation (rTMS) on the expression of BDNF, VEGF and Nestin in the ischemic hippocampus of rats with cerebral is-chemia. Methods: A total of 16 SD rats were randomly divided into model group, rTMS group, rTMS+H89 group and rTMS+normal saline(NS) group (n=4 in each group). The rats in rTMS+H89 group and rTMS+NS group were intracerebroventricular injected with H89 or NS respectively. Reperfusion models with middle cere-bral artery occlusion were established in the 4 groups. rTMS of 20 Hz was given to 3 groups except model group for 7 days. The expression changes of BDNF, VEGF and Nestin were investigated between model group and rTMS group, rTMS+H89 group and rTMS+NS group by Western Blot respectively. Results:Compared with model control group,the expressions of pCREB, BDNF, VEGF and Nestin were increased in the rTMS group ( <0.05). Compared with rTMS+NS group , the expressions of pCREB, BDNF, VEGF and Nestin were signifi-cantly decreased in the rTMS+H89 group ( <0.05). Conclusion:Repetitive transcranial magnetic stimulation of 20Hz could promote BDNF and VEGF expressions in the ischemic hippocampus after cerebral ischemia, which may contribute to the neurogenesis.