海南槌果藤叶提取物的抗氧化及抑菌活性研究

摘要

为了评价药用植物海南槌果藤的抗氧化活性及抑菌活性,本实验通过气相色谱与质谱联用技术(Gas chromatography-mass spectrometry,GC-MS)分析鉴定了海南槌果藤叶片甲醇提取物的化学成分,采用紫外-可见分光光度法测定了海南槌果藤甲醇提取物的总多酚、总黄酮含量、DPPH自由基、ABTS自由基、羟基自由基和超氧阴离子的清除作用;以及槌果藤氯仿相萃取物对三种食源性致病细菌和六种植物病原真菌的抑制作用.实验结果表明:海南槌果藤叶片甲醇提取物中含量最多的是酯类(45.03％)和杂环类(33.74％),其中丁基4-庚烷基-邻苯二甲酸酯(20.58％)为主要的单组分物质.海南槌果藤甲醇提取物中总多酚含量为1.93％,总黄酮含量为2.3％;进一步研究表明海南槌果藤甲醇提取物对DPPH自由基和ABTS自由基有一定的清除能力,其IC50分别为0.454 mg/mL和0.297 mg/mL;而对羟基自由基和超氧阴离子有较强的清除能力,IC50分别为0.937、2.261 mg/mL,分别比芦丁和TBHQ标准品低.除此之外,海南槌果藤氯仿相萃取物对三种食源性致病细菌和六种植物病原真菌均有一定的抑制作用,其中对革兰氏阴性菌(大肠杆菌和铜绿假单胞菌)抑制效果较明显;同时对葡萄溃疡病菌抑制作用最强,抑制率为43.97％,其次是新暗色柱节孢,抑制率为37.5％.%In order to evaluate the antioxidant activity and antibacterial activity of the medicinal plants Capparis zeylanica Linn.,the chemical constituents of the methanol extracts of C.zeylanicawere determined by GC-MS.The total polyphenols and flavonoids components,as well as the clearance rate of DPPH radical,ABTS radical,hydroxyl radical and superoxide anion radicalwere detectedwith UV-Visible spectrophotometricmethods.In addition,the inhibitory effect on three foodborne pathogenic bacteria and six plant pathogenic fungi were also determined.The results of GC-MS analysis showed that the major component of methanol extracts were esters (45.03％),followed by heterocyclic (33.74％) and butyl-4-heptyl-o-xylene acid ester (20.58％) was the main single component material.The total phenolics content of the methanol extracts was 1.93％ and the total flavonoid content was 2.3％.Further studies showed that the methanol extracts of C.zeylanica had certain scavenging ability on DPPH and ABTS free radicals,the IC50 were 0.454 mg/mL and 0.297 mg/mL,respectively,while the hydroxyl radical and superoxide anion were stronger and the IC50were 0.937 mg/mL and 2.261 mg/mL,respectively,which were lower than those of rutin and TBHQ.Furthermore,the chloroform phase extracts of C.zeylanica had certain inhibitory effectfor three food-borne pathogenic bacteria and six plant pathogenic fungi.The inhibitory rate of the chloroform phase extracts of C.zeylanica to Lasiodiplodia theobromae was the highest with 43.97％,followed by Neoscytalidium dimidiatum,the inhibition rate was 37.5 ％.