细辛苯丙素类化合物生物合成相关酶基因的挖掘及其表达分析

摘要

为了探明细辛苯丙素类化合物生物合成相关酶基因的表达水平,本研究基于实验室已构建好的细辛转录组数据库,根据基因功能注释筛选出与苯丙素类化合物生物合成相关酶基因,并推导其蛋白序列,进行生物信息学分析,同时对相关酶基因进行基因的表达分析.本文从细辛转录组数据库中筛选出了6个酶基因,分别为pal、c4h、comt、4cl、ccr、cad.基因编码蛋白特性分析结果表明:6个酶基因所编码的蛋白属于不同的超家族.基因表达分析结果表明:6个酶基因表达量呈现相同的变化规律.对于叶、叶柄、根、根茎,6个酶基因在花期的表达量较花前期的高;同时,6个酶基因在地下部分的表达量比地上部分的高.以上结果显示6个酶基因在细辛叶、叶柄、根、根茎的表达量差异明显,但具有一定的规律性.研究的成功开展为我们进一步了解苯丙素类化合物生物合成相关酶的功能和特性打下了基础,为今后细辛活性成分的生物合成及代谢调控提供理论依据.%In order to explore the expression level of enzyme genes relevant to the biosynthesis of phenylpropanoids in Asarum,the enzyme genes relevant to the biosynthesis of phenylpropanoids were screened out according to the gene functional annotation which was based on the database of Asarum transcriptome constructed in the laboratory.The protein sequence was deduced and was analyzed by bioinformatics.Meanwhile,expression analysis of the enzyme genes was carried out.Six enzyme genes were screened out from the transcriptome of Asarum,including pal,c4h,comt,4cl,ccr and cad.The protein sequence encoded by six enzyme genes had different superfamily.The expression level of six enzyme genes showed the same changes.For leaf,petiole,root and rhizome,the expression level of six enzyme genes in flowering stage was higher than that in early flowering stage,and the expression level of six enzyme genes in the underground part was higher than that in the aboveground part.The above results showed thatthe expression level of six enzyme genes in leave,petiole,root and rhizome of Asarum was different,but there was a certain regularity.The success of this study will lay a foundation for further understanding of the functions and characteristics of the enzymesrelevant to the biosynthesis of phenylpropanoids,and provide a theoretical basis for future biosynthesis and metabolic regulation of active ingredients in Asarum.