Molecular Cloning, Characterization and Expression Analysis of a Gene Encoding Hydroxyphenylpyruvate Reductase Involved in Rosmarinic Acid Biosynthesis Pathway from Perilla frutescens

摘要

In this study, a novel full-length cDNA of hydroxyphenylpyruvate reductase (HPPR) involved in rosmarinic acid (RA) biosynthesis pathway in Perilla frutescensis is cloned (designated as PfHPPR) for the first time by conserved fragment PCR and rapid amplification of cDNA ends (RACE). The cDNA of PfHPPR is 1723 bp long with an open reading frame (ORF) of 939 bp encoding a protein of 313 amino acid residues. Bioinformatics analysis revealed that the deduced amino acid sequence of PfHPPR has high homology to the other known HPPR proteins. Quantitative real-time PCR analysis of the transcription pattern indicates that the PfHPPR expresses constitutively in all examined tissues but most highly in leaf. Besides, the expression of PfHPPR can be induced by several factors such as abscisic acid (ABA), salicylic acid (SA), and ultraviolet-B radiation (UV-B).