基于玻璃毛细管制备用于生物检测的纳米孔

摘要

利用玻璃毛细管,发明了一种新型用于生物检测纳米孔的制备方法.实验表明,在玻璃毛细管内壁包埋石蜡层,通过加热玻璃微管的局部,可以拉制形成直径约50 nm的纳米孔.研究表明,制备玻璃纳米孔的关键步骤是控制吸附在内壁上的石蜡层的厚度,这直接影响纳米孔的直径.利用制备的纳米孔,我们已经成功地在实验中检测出生物分子,表明此种方法制作的玻璃纳米孔可以进行生物检测.通过这种方法可快速廉价地制作玻璃纳米孔,提供了一种替代硅技术制作纳米孔的技术.%A novel method of fabricating nanopores for biosensing is demonstrated.Experiment shows that the glass capillaries sealed with paraffin can be stretched to form a nanopore with the diameter of down to 50 nm.And the key step is to control the thickness of paraffin layer attached to the inner wall,which can affect the diameter of the nanopore.Bio-molecules were successfully detected in our experiment,which proves that the glass nanopores fabricated by this method are suitable for biosensing.Glass nanopores can be fabricated quickly and cheaply by this method,which will be a highly probable alternative to solid-state nanopores made by silicon nanotechnology.