基于rDNA序列分析的湖北克鲁维酵母系统发育地位探讨

摘要

The phylogenetic position of Kluyveromyces hubeiensis was investigated on the basis of 18S rDNA, internal transcribed spacer (ITS) region (including 5.8S rDNA) and 26S rDNA D1/D2 domain sequence analyses. The result indicated that K. hubeiensis is a distinct species closely related to Saccharomyces spencerorum and Kluyveromyces lodderae, with a closer relationship to the former. The close relationship between Kluyveromyces sinensis and Saccharomyces naganishii was also showed by the rDNA sequence analyses.%国内于20世纪90年代初曾描述过两个克鲁维酵母新种:中国克鲁维酵母(Kluyveromyces sinensis M. X. Li et al.)和湖北克鲁维酵母(Kluyveromyces hubeiensis M. X. Li et al.),二者均分自湖北神农架自然保护区.前者已被国际酵母菌分类学研究者接受和承认,而后者却一直被忽视.本文根据小亚基(18S) rRNA基因、大亚基(26S) rRNA基因D1/D2区和转录间区(ITS)序列分析,对K. hubeiensis进行了分子系统学研究.结果显示,K. hubeiensis代表一个具有充分分子系统学数据支持的独立种,该种与Saccharomyces spencerorum和Kluyveromyces lodderae形成一个高支持率的分枝,且与前者更近缘.本研究还显示,K. sinensis与Saccharomyces naganishii具有很近的亲缘关系.鉴于目前仅依靠序列分析对Kluyveromyces和Saccharomyces及其它相关属进行调整尚不现实,故建议仍维持这两个属的传统概念,并继续使用原种名.