A process for preparing a strain of the yeast Kluyveromyces, process for preparing a polypeptide in Kluyveromyces, the use of Kluyveromyces as a host, transformed Kluyveromyces cell shaped and Kluyveromyces expression vector and plasmids

摘要

The invention relates to yeasts of the genus Kluyveromyces, in particular K. lactis, acting as hosts for the expression of preprothaumatin or its various allelic and modified forms or their maturation forms, which yeasts contain an rDNA plasmid comprising a structural gene encoding for a thaumatin-like protein, an autonomous replicating sequence derived from K. lactis (KARS), a yeast regulon derived from the GAPDH-genes of S. cerevisiae, and a selection marker, e.g. the trp-1 or the lac-4 gene from S. cerevisiae and K. lactis, respectively. Optionally the plasmid also contains a transcription terminator from a yeast. Further the preparation of such yeasts and the preparation of thaumatin-like proteins with such yeasts and the proteins so obtained are claimed. The yeast described give a better expression than obtained with E. coli as described in EP-PA (A2) 0 054 330 and 0 054 331, published on 23 June 1982.