GENOME-SCALE METABOLIC NETWORK MODEL RECONSTRUCTION OF KLUYVEROMYCES MARXIANUS AND STRATEGIES FOR ENGINEERING NON-NATIVE PATHWAYS FOR 3-HYDROXYPROPIONATE PRODUCTION IN KLUYVEROMYCES MARXIANUS

摘要

One embodiment is related to Metabolic Network Model analysis metabolic characteristics kluyveromyces marxianus microorganism to generate 3-hydroxypropionate (3HP) and metabolic characteristic, analyzes the use of kluyveromyces marxianus. More specifically, embodiment is related to constructing the relationship that Metabolic Network Model kluyveromyces marxianus uses gene protein biochemical reaction, to the metabolic characteristic in analysis, using the model such as metabolic engineering, and for predicting which resource increment 3HP of new metabolic pathway using emulation based on metabolic engineering. The method of the embodiment of the present invention effectively predicts the microorganism of production capacity and vitro growth rates, saves time and cost, to optimize novel path, specific metabolite can be provided by providing variant microorganism. ;The 2014 of copyright KIPO submissions