专一转化人参二醇类皂苷Rb1为Rd的真菌菌株的筛选

摘要

于2009年的7-10月间在辽宁省的桓仁,吉林省的集安、靖宇、抚松等药材产区采集人参及人参根际土壤样品45份.通过真菌分离和培养,共获得真菌菌株105株,经形态学鉴定分属于15属48种.通过活性筛选,得到具有转化人参总皂苷活性的菌株25株,其中菌株SR87和SR105对人参皂苷Rb1具有专一转化活性.通过TLC和HPLC检测,其转化产物为人参皂苷Rd.经形态学鉴定,确定阳性菌株SR87为莫勒接霉Zygorhynchus moelleri,SR105为灰绿犁头霉Absidia glauca.这两株真菌均有较高的转化潜力,可以应用于制备人参皂苷Rd.%During July and October in 2009, 45 samples of ginseng roots and thizosphere soil were collected in medicinal plant growing areas of Liaoning and Jilin Provinces. A total of 105 fungal strains were isolated, and 48 species belonging to 15 genera were identified. Twenty-five strains showing positive activity of transforming total ginsenosides were screened. Among them strains SR87 and SR105 were found to have the specificity to transform ginsenoside Rb1 into ginsenoside Rd by using TLC and HPLC methods. Strain SR87 was identified as Zygorhynchus moelleri, and strain SR105 as Absidia glauca. They were shown to possess commercial potential for transformation of Rb1 into Rd.