蛹虫草菌丝产虫草素液体培养条件的研究

摘要

通过对蛹虫草菌丝产虫草素液体堵养条件的研究,明确蛹虫草菌丝产虫草素的适宜碳源及浓度,适宜氮源及浓度,最适pH值,最适培养温度,最适转速以及最适培养时间,以便应用于虫草素的工厂化生产.结果表明,蛹虫草菌丝产虫草素的条件:适宜碳源为D-果糖,最适浓度为10g/L;适宜氮源为蛋白胨,最适浓度为15g/L:最适初始pH为7,最适培养温度为24℃,最适转速为180r/min,最适培养时间为9d,其培养液虫草素含量可达到0.537g/L.%Liquid culture conditions for promoting Cordyceps militaris mycelia to secrete cordycepin were studied in order to understand the appropriate carbon and nitrogen sources and their suitable concentration, optimal pH value, cultivation temperature,shaking speed, and cultivation period. The purpose of this study is to apply these conditions to industrial production of cordycepin.Results showed that D-fructose and peptone are the appropirate carbon and nitrogen sources with an optimal concentration of 10g/L and 15g/L, respectively. The optimal initial pH, cultivation temperature, shaking speed and cultivation period were 7.0, 24℃,180r/min and 9 days respectively. By use of these culture conditions, the content of cordycepin in the culture fluid attained to 0.537g/L.