目的：优选威灵仙配方颗粒的提取工艺并建立威灵仙配方颗粒的定性鉴别。方法：以齐墩果酸、常春藤皂苷元及出膏率为考核指标，采用L9（34）正交试验考察威灵仙配方颗粒的提取工艺；采用薄层色谱法（TLC）进行定性鉴别。结果：威灵仙配方颗粒的最佳提取工艺为加12倍量水，浸泡0.5 h，煎煮1 h，提取3次；TLC鉴别特征斑点清晰，专属性强。结论：该提取工艺方法合理、操作简单，有效成分提取效率高，为本品生产和质量控制提供科学依据。%This study was aimed to optimize extraction process of formula granules of Clematis chinensis Osbeck and establish its qualitative discrimination method. With oleanolic acid, hederagenin and extract rate as indexes, extraction technology conditions of formula granules of C. chinensis Osbeck were investigated by L9 (34) orthogonal test method. TLC was used in the qualitative discrimination of formula granules of C. chinensis Osbeck. The results showed that optimum extraction technology was: adding 12 times of water; soaking for 0.5 h; cooking for 1 h; and extracting for 3 times. TLC had the diagnostic characteristic for the distinct spot which illustrated the specificity. It was concluded that the extraction process was reasonable, simple and feasible with high extraction efficiency of ef-fective contents, which can be the scientific basis for production and quality control of formula granules of C. chin-ensis Osbeck.
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