高效液相色谱法同时测定加味藿香正气软胶囊中橙皮苷、厚朴酚、和厚朴酚和甘草苷的含量

摘要

This study was aimed to develop an HPLC method for the determination of hesperidin,magnolol,honoki-oland liquiritin in Soft Capsule Jia-Wei Huo-Xiang Zheng-Qi (JWHXZQ). AKromasil C18 column (250 mmí4.6 mm, 5 μm) was used with water-methanol as mobile phasegradient elution. The flow rate was 1.0 mL·min-1, and the de-tecting wavelength was at 287 nm. The results showed that the linearity ranges ofhesperidin,magnolol,honokioland liquiritinwere 4.47-178.70 μg·mL-1, 3.42-136.96 μg·mL-1, 3.49-139.48 μg·mL-1, 3.92-157.20 μg·mL-1, respec-tively (r>0.999). The average recoveries of them were 99.48%, 99.05%, 99.57% and 99.79%, respectively. It was concluded that the method was accurate, sensitive and specific for quality control of Soft Capsule JWHXZQ.%目的：建立高效液相色谱法同时测定加味藿香正气软胶囊中橙皮苷、厚朴酚、和厚朴酚和甘草苷的方法。方法：采用Kromasil C18色谱柱（250 mm伊4.6 mm，5滋m），以水-甲醇为流动相梯度洗脱，流速1.0 mL·min-1，检测波长287 nm。结果：橙皮苷、厚朴酚、和厚朴酚、甘草苷分别在4.47-178.70滋g·mL-1、3.42-136.96滋g·mL-1、3.49-139.48滋g·mL-1、3.92-157.20滋g·mL-1范围内与峰面积呈良好线性关系（r=0.9999），平均回收率分别为99.48%、99.05%、99.57%、99.79%。结论：该方法准确、灵敏、专属性强，为加味藿香正气软胶囊质量标准提高奠定了基础。