牛奶和奶粉中维吉尼霉素残留量的液相色谱——串联质谱测定

摘要

The virginiamycin M1 residues in bovine milk and milk powder were determined with LC-MS-MS.This method solved the determination of virginiamycin M1 residues in bovine milk and milk powder.The calibration curve of cirginiamycin M1 showed good linearity in the range of 0.125μg/kg-8.00μg/kg with r=0.9998.The detection limit was 0.25μg/kg for bovine milk and 2.00μg/kg for milk powder.Spiked with standard at four levels(n=10),the samples had the recoveries of 62.04％-111.65％ for bovine milk and 85.49％-106.44％ for milk powder,the relative standard deviation was 8.455％-20.03％ for bovine milk and 2.467％-3.014％ for milk powder.%建立了牛奶和奶粉中维吉尼霉素virginiamycin M1残留量的液相色谱——串联质谱测定方法.结果表明,维吉尼霉素M1在0.125μg/kg～8.00μg/kg范围内,具有良好的线性关系,相关系数r=0.9998.牛奶中维吉尼霉素M1方法检出限为0.25μg/kg,奶粉中维吉尼霉素M1方法检出限为2.00μg/kg.在4个添加水平上进行回收实验(n=10),牛奶平均回收率为62.04％～～ 111.65％,相对标准偏差在8.455％～20.03％之间；奶粉平均回收率为85.49％～106.44％,相对标准偏差在2.467％～3.014％之间.