Objective To analyse the level of placental protein-13 (PP13)and placental growth factor (PIGF) of serum during 11+0~13+6 weeks of gestation, to predict the risk of pre-eclampsia in pregnant women with high risk. Methods Select-ed 208 cases from women with pregnancy at 11+0~13+6 weeks of gestation.Samples were tested for PP13 and PLGF by enzyme-linked immunosorbent assay. The pathogenetic condition and pregnancy outcome of those cases were observed. The sensitivity and specificity of PP13 and PLGF for the prediction of PE were analyzed through receiver operating characteristic (ROC) curve. Results Among 208 previously normotensive women,117 developed into pre-eclampsia,including 25 severe and 92 mild pre-eclampsia. 56 abnormal cases remained normal till the end of pregnancy were selected as control group. During 11+0~13+6 weeks of gestation,serum level of PP13 and PIGF of gravida with PE was (41.80±10.09)pg/ml,(45.88±16.58)pg/ml,which was sig-nificantly different from (125.06±31.30)pg/ml and (97.06±25.40)pg/ml of healthy gravida (P<0.05),ROC curve indicated that the sum of multiple of the median (MoM) of serum PP13 and PIGF level during the first trimester had high sensitivity (96.7%) and specificity (96.3%) for predicting the risk of PE. Conclusion Combined the detection of PP13 with PLGF might has good clinical value for detecting PE in pregnant women with high risk.%目的：分析孕11+0~13+6周子痫前期高危人群中胎盘蛋白13（PP13）与胎盘生长因子（PLGF）在血清中的表达，评估其对高危人群子痫前期（pre-eclampsia，PE）的预测意义。方法随机选择孕龄为11+0~13+6周的子痫前期高危妊娠妇女208例，取外周血，采用ELISA法检测血清PP13、PLGF的浓度，追踪受检者的晚期妊娠情况及分娩情况，用ROC曲线分析PP13、PLGF单独和联合预测PE发生的灵敏度与特异性。结果208例孕妇中，发展为子痫前期者117例，包括92例轻度子痫前期和25例重度子痫前期（其中5例为早发型重度子痫前期）；除产科其他并发症的异常妊娠情况，正常妊娠结局56例作为对照组。孕11+0～13+6周的PE患者外周血与高危妊娠妇女中妊娠结局正常的孕妇比较，PP13分别为（41.80±10.09）pg/ml、（125.06±31.30）pg/ml，PLGF分别为（45.88±16.58）pg/ml、（97.06±25.40）pg/ml，差异有统计学意义（P<0.05）。 ROC曲线结果显示孕11+0~13+6周妊娠妇女的PP13、PLGF中位数倍数（MoM）之和预测PE的发生具有较高的灵敏度（96.7%）和特异性（96.3%）。结论在子痫前期的高危妊娠妇女中，PP13与PLGF联合检测对子痫前期的发生有较好的预测价值。
展开▼
