夏枯草提取物对α-淀粉酶抑制作用的初步研究

摘要

胃的探讨夏枯草提取物对α-淀粉酶的抑制作用.方法 采用水提和水提醇沉方法 得到夏枯草水提物、浸膏、粗多糖,通过Van Loon's碘比色法检测α-淀粉酶活性,以夏枯草水提物测定该酶动力学.结果 夏枯草水提物对α-淀粉酶有抑制作用,呈量效关系.对该酶抑制类型为反竞争性抑制.夏枯草浸膏对α-淀粉酶抑制作用较水提物明显.结论夏枯草水提物、浸膏对α-淀粉酶存在抑制作用,有效部位可能在浸膏部分,为深入研究夏枯草提取物抑制单糖吸收作用提供了依据.%Objective It is to explore the inhibiting function of extract from Prunella vulgaris L. on α- amylase(α- AL). Methods Aqueous extract, polysaccharide and aqueous-ethanol extract was obtained from Prunella vulgaris L. by aqueous ex-tract and ethanol extract methods. The activity of α- AL was determined through Van Loon's method, using aqueous ex-tract from Prunella vulgaris L. to determine its enzyme kinetics. Results Aqueous extract from Prunella vulgaris L. could in-hibit the activity of α- AL, and there was a doee-effect relationship between them. The inhibiting type of this amylase was uncompetitive inhibition. The effect of aqueous extract from Prunella vulgaris L. on a - AL was more obvious than that of. aqueous-ethanol extract. Conclusion Aqueous extract and aqueous-ethanol extract from Prunella vulgaris L. has inhibiting ef-fect on α- AL,and the utility part of Prunella vulgaris L. was aqueous-ethanol extract, which provides an evidence to do fur-ther study on inhibiting glucose absorption of Prunella vulgaris L.