乳腺浸润性导管癌组织中p－AKT、VEGF－C的表达及其与侵袭、转移的关系

摘要

目的：探讨乳腺浸润性导管癌（IDC）组织磷酸化蛋白激酶B（p－AKT）、血管内皮细胞生长因子－C（ VEGF－C）表达水平，及其与淋巴结侵袭转移的关系。方法应用免疫组织化学法检测72例乳腺IDC组织及30例乳腺腺瘤组织中p－AKT、VEGF－C的表达水平，分析p－AKT与血管生成的关系。结果乳腺IDC组织中VEGF、p－AKT蛋白的阳性表达率分别为59％和67％，均显著高于乳腺腺瘤组织中20％和17％的阳性表达率（χ2＝13.39，21．21，P＜0．05）；与有淋巴结转移者比较，无淋巴结转移的乳腺IDC组织VEGF－C、p－AKT蛋白阳性表达率显著降低（χ2＝11．10，5．51，P＜0．05）；乳腺IDC组织VEGF－C、p－AKT蛋白表达水平呈显著正相关（r＝0．823，P＜0．05）。结论乳腺IDC组织中VEGF－C、p－AKT呈过度表达状态，共同参与淋巴结的侵袭与转移，阻断PI3K／AKT通路有望为乳腺IDC的靶向治疗提供一种新的思路。%Objective It is to investigate the expression of vascular endothelial growth factor (VEGF), protein kinase B (P-AKT) in breast invasive ductal carcinoma (IDC), and its relation with lymph node invasion and metastasis.Methods The expression of p-AKT, VEGF-C in the tissue of 72 cases of breast IDC and 30 cases of breast fibroadenoma were detec-ted by immunohistochemical staining, the correlation between p-AKT and angiogenesis were analyzed.Results The positive expression rate of p-AKT, VEGF-C protein in breast IDC was 59%and 67%respectively, which was significantly higher than that in breast fibroadenoma of 20%and 17%respectively (χ2 =13.39, 21.21, all P<0.05).The expressions of p-AKT, VEGF-C in breast IDC with lymphnode metastasis were higher than those without lymphnode metastasis (χ2 =11.10, 5.51, all P<0.05).The positive expression rate of VEGF-C had significant positive correlation with that of p-AKT (r=0.823, P<0.05).Conclusion The expression of p-AKT, VEGF-C in breast IDC is over-expressive, and participate the lymphnode invasion and metastasis.Inhibition of PI 3K/AKT signal transduction pathway can be expected to provide a new method for the target therapy of breast IDC.