千金藤素对骨肉瘤MG-63细胞迁移、侵袭能力影响的体外研究

摘要

Objective It is to study the effect of Cepharanthine on migration and invasion of osteosarcoma MG -63 cells in vitro, and explore the related mechanism.Method s The cultured MG-63 cells were divided into 10umol/L Cepharanthine group, 40umol/L cepharanthine group and control group.Wound healing assay and Transwell chamber method were used to de-tect the migration and invasion respectively.The level of the expression of matrix metal enzyme-2 was detected by enzyme linked immunosorbent assay ( ELISA).Results The migration of the cells in 10umol/L Cepharanthine group, 40umol/L cepharanthine group to the scratch area after 24 hours significantly reduced, with cepharanthine concentration increased, cell migration to scratches significantly reduced.The number of penetrating cells were less in cepharanthine groups than that in control group, and the numbers in 40 umol/L cepharanthine group were less than that in 10umolL/Cepharanthine group.The level of MMP-2 expression of MG-63 cells decreased with the increase of Cepharanthine concentration and extension of treat time.The concentrations of MMP-2 in cell culture solution were lower in cepharanthine groups than that in control group , and the concentration in 40umol/L cepharanthine group were less than that in 10umolL/Cepharanthine group, the differences were all significant (P<0.05).Conclu sion Cepharanthine could inhibit the migration and invasion of the osteosarcoma MG-63 cells in vitro.The inhibitory effect may be achieved by inhibiting the expression of MMP-2 in tumor cells.%目的 探讨千金藤素对骨肉瘤MG-63细胞迁移和侵袭能力的影响及其相关机制.方法 将培养的MG-63细胞分为10μmol/L千金藤素组、40μmol/L千金藤素组和对照组,观察3组划痕迁移试验、Transwell小室侵袭试验骨肉瘤MG-63细胞迁移、侵袭能力,用酶联免疫吸附试验检测3组基质金属蛋白酶-2(MMP-2)浓度.结果 10μmol/L千金藤素组、40μmol/L千金藤素组细胞向划痕处迁移明显减少,且随着千金藤素浓度的增加,细胞向划痕处迁移明显减少.10μmol/L千金藤素组、40μmol/L千金藤素组穿膜细胞数明显少于对照组(P均<0.05),且40μmol/L千金藤素组明显少于10μmol/L千金藤素组.骨肉瘤MG-63细胞培养液中MMP-2浓度随着作用时间的延长而降低,10μmol/L千金藤素组、40μmol/L千金藤素组培养24 h、48 h、72 h的MMP-2浓度均明显低于对照组,且40μmol/L千金藤素组均明显低于10μmol/L千金藤素组,差异均有统计学意义(P均<0.05).结论 千金藤素可抑制骨肉瘤MG-63细胞迁移和侵袭能力,这种抑制效应可能是通过抑制肿瘤细胞MMP-2的表达来实现的.