补肾通络方对膝骨关节炎大鼠氧自由基的影响

摘要

Objective It is to observe the effect of Bushen Tongluo decoction on the levels of SOD,MDA and LPO in serum of rats with knee osteoarthritis.MethodS 42 Wistar rats were randomly divided into normal group, model group, western medicine group,proprietary Chinese medicine group,low,medium and high dose of Bushen Tongluo groups,each group had 6 rats.The rats in the normal group was given no treatment,the rats in the other groups were given drugs into knee joints by injection to establish model of knee osteoarthritis.After the models were established successfully the rats in every treatment group were given the respective drugs by gavage,the ones in normal group and model group were given the same dose of normal saline.The contents of SOD,MDA and LPO in serum were detected by enzyme linked immunosorb-nent assay(ELISA)in every group after treatment for 4 weeks.ReSultS The serum contents of MDA and LPO in every treatment group were lower while the content of SOD in western medicine group and proprietary Chinese medicine group were higher than those in model group(P<0.05), no difference in SOD was found among Bushen Tongluo groups and model group(P>0.05).There was no significant difference in serum contents of MDA, LPO and SOD among Bushen Tongluo groups(P>0.05).The serum content of MDA in high dose of Bushen Tongluo group was higher than that in west -ern medicine group(P<0.05), that of LPO was higher than those in western medicine group and proprietary Chinese medicine group(P<0.05).ConcluSion Bushen Tongluo decoction can inhibit the production of MDA and LPO and pro-mote the secretion of SOD,thus to treat for knee osteoarthritis.%目的 观察补肾通络方对膝骨关节炎大鼠血清中超氧化物歧化酶(SOD)、丙二醛(MDA)和过氧化脂质(LPO)的影响.方法 将42只Wistar大鼠随机分为正常组、模型组、西药组、中成药组、补肾通络方低剂量组、补肾通络方中剂量组、补肾通络方高剂量组,每组6只.正常组大鼠不予任何处理,其余组大鼠按照膝关节内注射药物的方法复制膝骨关节炎模型.造模成功后各给药组分别灌胃相应药物,正常组和模型组灌胃等体积蒸馏水.连续灌胃4周后用酶联免疫吸附(ELISA)法检测各组大鼠血清中SOD、MDA、LPO含量.结果 各给药组血清MDA和LPO含量均明显低于模型组(P均<0.05),西药组和中成药组SOD含量均明显高于模型组(P均<0.05),补肾通络方各剂量组SOD含量与模型组比较差异无统计学意义(P均>0.05).补肾通络方各剂量组血清MDA、LPO、SOD含量比较差异均无统计学意义(P均>0.05);补肾通络方高剂量组血清MDA含量明显高于西药组(P<0.05),LPO含量明显高于西药组和中成药组(P均<0.05).结论 补肾通络方可以通过抑制血清中MDA、LPO的含量及增加SOD的含量,起到治疗膝骨关节炎的作用.