秋刀鱼中组胺菌的分离与鉴定

摘要

In order to analyze the species of histamine forming bacteria（HFB）in Saury fish,isolation and identification of HFB in saury fish were performed by using histidine broth media to isolate strain,the enzymes and the physiological and biochemical assay,electrophoresis technique for histamine assay and the bacteria identification kit.The results showed that one HFB was isolated from saury fish,the strain was identified to be Pseudomonas spp based on biochemistry experiment and the morphology properties,bacteria identification kit.Histamine increased gradually with temperature increasing in the sample inoculated with this strain which stored at 5 ℃ to 30 ℃,it was detected at the most level of about 2200 mg/kg in the sample inoculated with the strain which stored at 30 ℃.%通过半海水寒天培养基及组胺酸肉汤选择性培养液,组胺的电泳分析技术,对秋刀鱼肉中的组胺生成菌进行筛选、理化分析和功能酶检测及细菌鉴定试剂盒API20E的分析试验,进而对秋刀鱼肉中的组胺菌进行分离鉴定。结果分离得到1株组胺生成菌,通过生化分析,此菌株为假单胞菌。此菌株在5～30℃的培养温度范围内,随温度升高,组胺生成量逐渐增加,在30℃有最高组胺生成量,约2 200mg/kg。