Objective To detect the expressions of BAFF, BAFF-R and Bcl-xL in peripheral blood from patients with B cell non-Hodgkin's lymphoma ( B-NHL ), and to investigate the correlation between the level of these genes and the progression of B-NHL. Methods The expression of BAFF, BAFF-R and Bcl-xL in peripheral blood mononuclear cells ( PBMC ) from patients ( n = 36 ) and healthy volunteers ( n = 7 ) was examined via real-time fluorescence quantitative polymerase chain reaction ( real-time q-PCR ) at the mRNAs level. These data were further analyzed according to gender, lymphoma international prognostic index( IPI ), age, clinical staging and drug resistance. Results The expressions of BAFF, BAFF-R and Bcl-xL were significantly increased in PBMC from patients with B-NHL compared with control group ( P < 0.05 ). The expression of these genes in groups of stage Ⅲ/Ⅳ and the resistant B-NHL was respectively higher than that of stageⅠ/Ⅱ and non resistant B-NHL groups ( P <0.05 ). Conclusion The expression of BAFF, BAFF-R and Bcl-xL in PBMC from patients with resistant B-NHL is significantly increased, correlated with the B-NHL clinical staging. These results suggest that BAFF/BAFF-R mediated signal pathway is involved in the progression of B-NHL and drug resistance.%目的 研究B细胞性非霍奇金淋巴瘤(B-NHL)患者外周血B ceil-activating factor(BAFF)、其受体BAFF-R及Bcl-xL基因的表达,探讨其与B-NHL疾病进展的相关性.方法 应用实时荧光定量PCR法检测36例B-NHL患者外周血单个核细胞(PBMC)BAFF、BAFF-R、Bcl-xL mRNAs的相对表达水平,以7例健康献血员作为对照,并据性别、淋巴瘤国际预后指数(IPI)、年龄、临床分期、是否耐药进行分组比较.结果 B-NHL患者PBMC BAFF、BAFF-R及Bcl-xL的mRNAs表达较正常对照组显著升高(P<0.05),Ⅲ/Ⅳ期高于I/Ⅱ期,耐药B-NHL组高于非耐药组(P<0.05).结论 外周血单个核细胞BAFF、BAFF-R、Bcl-xL mRNAs的表达水平与B-NHL临床分期有关,在耐药B-NHL外周血中明显升高,提示BAFF/BAFF-R介导的信号通路可能参与B-NHL的临床进展及耐药机制.
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