法匹拉韦在细胞水平上对犬瘟热的抑制作用初探

摘要

目的 研究法匹拉韦(T-705)在细胞水平上对犬瘟热病毒(CDV)增殖的抑制效果.方法 利用间接免疫荧光实验及50％终点法测定犬源CDV-11株与貂源CDV-3株在Vero细胞和DH82细胞中的生长曲线.采用CCK-8法测定T-705对Vero细胞及DH82细胞活力的影响;测定在不同时间加入不同浓度T-705对CDV的抑制效率.结果 细胞毒性实验表明,T-705对Vero细胞的活性具有轻微抑制作用,对DH82细胞的活性基本无影响.在2.441～1250μg/ml实验范围内,T-705可有效抑制CDV-3和CDV-11在Vero及DH82细胞中的增殖.在细胞感染病毒后的不同时间点(12,24,36,48 h)加入T-705,仍可产生持久的抗病毒效果.结论 T-705可在细胞水平高效抑制CDV的增殖,是治疗犬瘟热的候选药物.%Objective To investigate the inhibitory effect of favipiravir (T-705) on canine distemper virus (CDV) replication in Vero cells and DH82 cells.Methods The growth curves of CDV-11 strains from canine and CDV-3 strains from mink in Vero cells and DH82 cells were determined with indirect immunofluorescence assay and 50％ endpoint titration.The viability of Vero and DH82 cells was determined using the Cell Counting Kit-8.CDV inhibition at different concentrations of T-705 at different time points was measured .Results Cytotoxicity data showed that there was a moderate decline of viability in Vero cells after T-705 treatment, but no apparent cytotoxicity in DH 82 cells.T-705 significantly inhibited the replication of CDV-3 and CDV-11 in both Vero cells and DH82 cells in the test range of 2.441-1250 μg/ml. T-705 exhibited effective and stable antiviral activity when given at different time points post virus challenge .Conclusion Our results demonstrate that T-705 has effective antiviral activity and may be a promising anti-CDV drug candidate .