葛根素对急性酒精性肝损伤的预防作用

摘要

目的：观察葛根素对急性酒精肝损伤的预防作用。方法将32只SD大鼠按随机数字表法分为4组（空白组、模型组、葛根素高剂量组、葛根素低剂量组），每组8只。葛根素高剂量组给予葛根素0．8 g／kg，葛根素低剂量组给予葛根素0．4 g／kg，连续给药30 d，空白组及模型组给用同等计量的蒸馏水。解剖前，模型组及葛根素高、低剂量组按14 mL／kg体积（56°白酒）经口灌胃，建立急性酒精肝损伤模型；造模后禁食16 h，处死，检测血清中三酰甘油（TG）、丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（ AST）水平变化，观察肝脏病理学组织变化。结果模型组大鼠血清中 TG、ALT、AST均高于空白组（ P ＜0．05），葛根素低剂量组、葛根素高剂量组大鼠血清中 TG、ALT、AST 均低于模型组（P＜0．05），且与空白组比较差异无统计学意义（P＞0．05）。空白组、模型对照组、葛根素高剂量组、葛根素低剂量组的肝质量／体质量分别为0．0332±0．0011、0．0390±0．0051、0．0348±0．0024、0．0338±0．0019，其中模型对照组高于空白组、葛根素高剂量组、葛根素低剂量组（P＜0．05），葛根素高剂量组、葛根素低剂量组与空白组比较差异无统计学意义（ P＞0．05）。结论葛根素可以预防大鼠急性酒精性肝损伤，对肝脏具有保护作用。%Objective To observe the preventive effect of puerarin on acute alcoholic liver injury . Methods Total of 32 SD rats were divided into 4 groups according to the random number table method (blank control group,model group,puerarin high dose group,puerarin low dose group),8 rats/group.The puerarin high dose group was given 0.8 g/kg puerarin,the puerarin low dose group was given 0.4 g/kg puer-arin,the blank group and model group were given the same measurement of distilled water,continuously for 30 d.Before dissection,the model group and puerarin high and low dose group were given 14 mL/kg volume (56 degrees liquor) by oral gavage,to establish acute alcoholic liver injury model;after making the model, 16 h fasting was done,and all groups were killed,and the serum triglyceride(TG),alanine aminotransferase (ALT),aspartate aminotransferase(AST) content changes,liver tissue pathology changes were observed. Results The serum TG,ALT,AST of the model group were higher than the control group(P<0.05),and TG,ALT,AST of the puerarin low-dose group, puerarin high dose group were lower than the model group (P<0.05),and had no statistically significant differences from the control group(P >0.05).The liver weight/body weight of the control group,model group,puerarin high dose group,puerarin low dose group were 0.033 2 ±0.001 1,0.039 0 ±0.005 1,0.034 8 ±0.002 4,0.033 8 ±0.001 9 respectively,the model group higher than the blank control group,puerarin high dose group,and puerarin low dose group(P<0.05),while the differences between the puerarin high dose group,puerarin low dose group and the control group were not statistically significant(P>0.05).Conclusion Puerarin can prevent acute alcoholic liver injury in rats, presenting a protective effect on the liver.