miR-29c靶向调控AMPK/mTOR信号通路对胃癌细胞生物学行为的影响

摘要

目的 探讨miR-29c靶向调控AMPK/mTOR信号通路对胃癌细胞生物学行为影响的机制.方法qPCR检测不同胃癌细胞株中miR-29c的表达情况;免疫荧光检测miR-29c-mimic转染胃癌SGC-7901细胞的转染效率;MTT增殖实验检测miR-29c对胃癌SGC-7901细胞增殖能力的影响;Transwell侵袭实验检测miR-29c对胃癌SGC-7901细胞侵袭能力的影响;Western blotting检测过表达miR-29c后p-AMPK和PRR5蛋白表达情况的变化.结果胃癌SGC-7901细胞中miR-29c的表达水平低于胃癌MGC-803、AGS和HGC-27细胞(P<0.05).转染72 h后,miR-29c-mimic可以有效地转染进入胃癌SGC-7901细胞.miR-29c-mimic组细胞增殖率以及p-AMPK和PRR5蛋白的表达水平明显低于NC组(P<0.05),迁移至膜下层的细胞数量明显少于NC组(P<0.05).结论miR-29c可能靶向AMPK/mTOR信号通路调控胃癌SGC-7901细胞的生物学行为.%Objective To investigate affecting mechanism of miR-29c targeting AMPK/mTOR signaling pathway on biological behavior of gastric cancer cells. Methods The miR-29c expressions in different gastric cancer cell lines were detected by quantitative polumerase chain reaction ( qPCR) . Immunofluorescence was used to detect transfection efficiency of miR-29c-mimic transfecting SGC-7901 gastric cancer cell. MTT proliferation assay was used to detect effect of miR-29c on proliferation ability of SGC-7901 gastric cancer cell. Transwell invasive test was used to detect effect of miR-29c on inva-sion ability of SGC-7901 gastric cancer cell. Western blotting was used to detect changes of p-AMPK and PRR5 protein ex-pressions after miR-29c overexpression. Results The miR-29c expression in SGC-7901 gastric cancer cell was significantly lower than those in MGC-803, AGS and HGC-27 gastric cancer cells (P<0. 05). At 72 h after transfection, miR-29c-mimic could effectively transfect into SGC-7901 gastric cancer cell. Values of cell proliferation rate and expression levels of p-AMPK and PRR5 proteins in miR-29c-mimic group were significantly lower than those in NC group (P<0. 05), while number of cells migrated to the submucosa was significantly less than that in NC group (P<0. 05). Conclusion miR-29c may target AMPK/mTOR signaling pathway to regulate biological behavior of gastric cancer cells.