目的:分析血小板(PLT)复检常见原因,探讨血细胞分析仪计数血小板过程中的相关影响因素,排除因假性增高和降低而导致的与临床不符的血小板结果,分析原因并处理,最终为临床提供准确的PLT检测结果.方法: 对使用XE-2100血细胞分析仪测得的350例PLT异常结果及血小板直方图异常的标本进行复检,采取重新采集血标本或手工计数及涂片瑞氏染色进行复检,以便查明原因.结果:350例血小板结果异常标本经分析常见复检原因为血小板异常偏高或降低,与临床不符,与直方图不符等原因均应复检.而标本放置时间,标本的混匀程度,血小板聚集,血小板体积,抗凝剂种类及比例,采血过程是否顺利等因素是导致血小板结果假性增高及降低的主要原因.结论:血小板计数不可完全依赖于血小板分析仪,对于异常PLT结果应进行复检,同时进行手工计数及涂片染色镜检的方法复检,方可发出报告,从而为临床提供可靠的PLT结果.%Objective:Analyze the main purposes for Platelet (PLT) re-inspection. Discuss the relevant factors which are impact blood cell analyzer count the Platelet, rule out the wrong PLT data that are not conform to clinical test results, which is caused by pseudo increased or reduce, then analyze the main reason and solve it, in order to provide the correct PLT-inspection data for clinical diagnosis.Methods: Inspect 350 abnormal PLT and PLT histogram specimens, which are measured by XE-2100 PLT analyzer. Collect PLT specimens again, or manual count, or Smear's staining to re-inspection, then find out factors which is cause the abnormal Platelet's test results.Results: Analyze the 350 abnormal PLT and PLT histogram specimens, find that the main reason for PLT re-inspection are abnormal PLT increase or decrease. And test result which is not conform to clinical diagnosis or Smear's staining, should take the PLT re-infection. And the main reasons of PLT pseudo increase or decrease are storage time of PLA specimen, specimen's mixed degree, PLT aggregation, PLT volume, anticoagulants species and proportion, and whether the blood collecting is successful.Conclusions:PLT counting can't rely on PLT analyzer absolutely, and should re-inspect the abnormal PLT data, and collect PLT pecimens or manual count at the same time, then submit the report. in order to provide reliable PLT data for clinical diagnose.
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