免疫印迹法和磁微粒化学发光法检测SLE自身抗体的比对分析

摘要

目的：比较免疫印迹法（Western blot）和磁微粒化学发光法(Magnetic particle Chemiluminescence method)检测系统性红斑狼疮（SLE）自身抗体的一致性。方法：用免疫印迹法和磁微粒化学发光法对123份血清进行检测，其中系统性红斑狼疮阳性患者63例，健康体检者60例，用卡方检验对两种方法结果进行比较分析。结果：免疫印迹法和磁微粒化学发光法中抗Sm抗体阳性率分别为27.0%和14.3%、抗RNP/Sm阳性率分别为54.0%和42.9%、dsDNA阳性率分别为41.3%和30.2%、抗核小体抗体阳性率分别为31.7%和20.6%、抗核糖体P蛋白抗体阳性率分别为28.6%和11.1%。两种方法的准确性无显著性差异（＞0.05）总符合率为80.6%，Kappa值为0.669。结论：免疫印迹法和磁微粒化学发光法检测系统性红斑狼疮自身抗体的检测结果具有可靠性，较好的符合率和准确性，磁微粒化学发光法可以作为简便、快速、经济的检测方法应用于系统性红斑狼疮自身抗体的检测。%Objective: The present study aims to compare Western blot and Magnetic particle Chemiluminescence method consistency on autoimmune antibody detection of systemic lupus erythematosus. Methods: Western blot assay and Magnetic particle Chemiluminescence method were used to detect autoimmune antibody from 123 serum samples, of which 63 patients with systemic lupus erythematosus, the others from 60 healthy persons. The results of the two methods were compared by X2 test. Results: Anti-Sm antibody positive rate of Western blot and Magnetic particle Chemiluminescence method were 27.0 and 14.3 percent respectively. Anti- RNP/Sm antibody positive rates were 39.7% and 42.9% respectively. Anti- ds- DNA antibody positive rates were 41.3% and 30.2% respectively. Anti- nucleosome antibody positive rates were 31.7% and 20.6% respectively. Anti- ribosomal protein antibody positive rates were 28.6% and 11.1% respectively. The accuracy of the two methods has no significant difference ( >0.05) . Conclusion: Western blot and Magnetic particle Chemiluminescence method antibodies has consistency in detecting autoimmune antibody from systemic lupus erythematosus, so the Magnetic particle Chemiluminescence method can be applied to detect autoantibody for its simple, fast, economical reason.