目的：探索肝脏移植并FK506联合用药后人血清中蛋白质组的变化，研究免疫排斥反应的机制及FK506联合用药的作用。方法取5例肝癌患者移植前和移植后24 h、10 d、30 d、90 d、180 d的血清样品，用Aurum Serum Protein Mini Kit除高峰度蛋白，进行双向电泳（2-DE）分离，ImageMaster 2D Platinum图像分析软件分析电泳图谱。结果建立移植前和移植后并FK506联合用药的人血清双向电泳差异图谱，蛋白斑点数在（877±59）～（897±61），移植前、后不同组之间的匹配均＞70％。共筛选出重复性好且表达显著差异的蛋白质斑点7个。结论初步建立了肝移植并FK506联合用药后人血清蛋白质组的2-DE分析方法。%Objective To analyze the serum proteome change after liver transplantation and FK 506 combination in human ser-um by two-dimensional gel electrophoresis (2-DE) and to study the mechanism of immune rejection and the mechanism of action of FK506 combination.Methods The serum samples of 5 liver transplantation patients pre-and post transplantation at 24 hours,10days,30 days,90 days 180 days were treated using Aurum Serum Protein Mini Kit , and than analyzed by 2-DE.The electrophoresis imageswere analyzed by using ImageMaster 2D platirum imaging analysis software.Results The differential maps of 2-DE of serum proteomebefore and after liver transplantation and FK506 combination were established.Protein spots between (877 ±59.65)-(897.6 ±61.01),the matching rate before transplantation and different groups after transplantation >70%.Among them 7 protein spots were selectedwith good repeatability and significant differential expression .Conclusions The 2-DE analytical method of serum proteome afterliver transplantation and FK506 combination was preliminarily established .The differential expression protein spots found in the studyhave laid a foundation for the further study .
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