HMGB1基因多态性与HBV相关性肝癌的关联性研究

摘要

Objective:To study the possible association of the genetic polymorphism of high mobility group protein B1(HMGB1)1176 G/C intron 4 with the susceptibility to hepatocellular carcinoma(HCC)after hepatitis B virus(HBV) infection. Method:110 patients with HBV-related HCC and 316 patients of HBV infection without HCC after HMGB1 intron4 1176G/C polymorphism were detected by polymerase chain reaction-restriction fragment length pdymo-rphism (PCR-RFLP)method,chi-square test and unconditioned logistic regression model were applied to analysis results. Result:There were significant statistically difference in the three genotypes among the HBV-related HCC group and the chronic HBV infection group(χ²=6.152,P=0.046;χ²=5.605,P=0.018). There were significant difference(P=0.023)between HCC and CHB under the dominant model either,hepatitis group of AsC,AsC+CHB different from HCC group(P=0.048,0.028) under the recessive model respectively. There were significant difference between HCC and AsC+CHB under codominant model(P=0.03). Conclusion:The results suggested that the genotype of HMGB1 intron4 1176 G/C is associated with the susceptibility to HBV-induced hepatocellular carcinoma.%目的：探讨高迁移率族蛋白B1（HMGB1）第4内含子1176G/C与HBV感染后肝癌是否存在关联。方法：采用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）方法检测110例HBV感染后肝癌患者及316例HBV感染后非肝癌患者HMGB11176G/C多态性，采用字2检验及非条件logistic回归统计方法进行分析。结果：乙肝肝癌组3种基因型及G、C等位基因分布与慢性乙型肝炎组比较差异有统计学意义（χ²=6.152，P=0.046；χ²=5.605，P=0.018）。在显性模式下乙肝肝癌组与慢性乙型肝炎组比较差异有统计学意义（P=0.023），在隐性模式下与乙肝病毒携带组、轻型肝病组比较差异有统计学意义（P=0.048，P=0.028），在共显性模式下与轻型肝病组比较差异有统计学意义（P=0.03）。结论：HMGB1基因多态性与HBV感染后肝癌易感性相关。