NDV7793促进人CD4+T细胞表达TRAIL的实验研究

摘要

Objective:To study the TRAIL up-regulation in human CD4+T cells which stimulated by NDV7793. Method:Pure CD4+T cells were isolated by using MACS. The CD4+T cells were pre-activated with anti-CD3/anti-CD28 antibodies and IL-2 at first. And then after pre-activated CD4+T cells were stimulated by NDV7793,the expression level of TRAIL was detected by FCM.Result:The purity of CD4+T cells was(97.38±0.28)%. The CD25,CD69 cells accounted for(29.30±1.08)%after activated which was higher than that(2.40±1.30)%of the negative control group. FCM results indicated,the expression of TRAIL increased significantly when CD4+T cells stimulated by NDV7793 compared with the control group,and reached the highest when NDV7793 at 25 HU.Conclusion:NDV7793 can up-regulate the expression of TRAIL and IFNγof pre-activated CD4+T cells.%目的：研究新城疫病毒（Newcastle disease virus，NDV）弱毒株NDV7793能否促进人CD4+T细胞表达肿瘤坏死因子相关凋亡因子诱导配体（TNF-related apoptosis-inducing ligand，TRAIL）。方法：首先用免疫磁珠分选法（magnetic activated cell sorting，MACS）分选外周血静止CD4+T细胞，然后加抗CD3抗体、抗CD28抗体和白细胞介素-2（interleukin-2，IL-2）使之成为能被NDV激活的CD4+T细胞。用流式细胞技术（flow cytometry， FCM）检测NDV刺激的CD4+T细胞的TRAIL的表达水平。结果：MACS法分选外周血得到的CD4+T细胞纯度达到（97.38±0.28）%；能被NDV激活的CD4+T细胞表面分子CD25和CD69双阳性表达率可达（29.30±1.08）%，与PBS阴性对照组（2.40±1.30）%相比，差异有统计学意义（P<0.05）；FCM检测结果显示，与对照组比较， NDV7793刺激的CD4+T细胞TRAIL表达水平均有显著升高，且在NDV7793效价为25 HU时达到最大值。结论：NDV7793可刺激CD3抗体、CD28抗体和IL-2预先活化的CD4+T细胞表达TRAIL。