组织工程骨支架制备及实验工艺参数对成型质量影响研究

摘要

The preparation of tissue engineering scaffolds based on piston extrusion deposition method can achieve a variety of ma-terials,speed controllable,high-precision preparation process.The materials used are hydroxyapatite(HA),polyvinyl alcohol(PVA) and suitable amount of silk fibroin(SF)joined.Certain concentrations of PVA material were prepared according to HA :SF ratio of 4:1 to join silk fibroin,so as to improve bone scaffold biocompatibility.The influence of a serious of process parameters such as the relationship among the velocity of the piston,the extrusion speed of the nozzle and the velocity of the moving platform,the height of the moving platform,and the ratio of HA,PVA and SF on the forming quality of bone scaffolds was analyzed.Through the experimental verification,the problems of forming materials piled up, spiraling, drawing and breaking of the molding material during the forming process are solved.Through the reasonable proportioning,the surface crack and the material collapse phenomenon of the wire material will not appear during the forming process.%基于活塞挤压沉积成型方式制备组织工程骨支架,能够实现多种材料、速度可控、高精度的制备过程.使用材料为羟基磷灰石(HA)、聚乙烯醇(PVA)及加入适量丝素蛋白(SF),制备确定浓度聚乙烯醇材料,制备时按照HA:SF为4:1比例加入丝素蛋白,提高骨支架生物相容性.分析活塞运动速度、喷嘴挤出速度及运动平台速度的相互匹配,喷嘴距离运动平台高度,羟基磷灰石(HA)、聚乙烯醇(PVA)和丝素蛋白(SF)的配比等一系列工艺参数对于骨支架成型质量的影响.通过实验验证,解决了成形过程中成型材料堆积、呈螺旋状、拉丝及断丝问题,通过合理配比,在成型过程中不会出现丝材表面裂纹及材料坍塌现象.