酱香大曲中产中性蛋白酶嗜热细菌的筛选及鉴定

摘要

In the experiments, 15 thermophilic bacterial strains were isolated from high-temperature Daqu. The activity of the produced neutral protease by those 15 strains were measured. And strain FBKL1.016 had the highest neutral protease activity (up to 68.3 U/g). Based on the indi-vidual morphological characteristic, physiological and biochemical experiments and molecular identification, it was identified as Bacillus li-cheniformis. This study provided research base for further exploration of the styles of Jiangxiang Baijiu(liquor).%通过对成品高温大曲进行嗜热细菌的分离培养,筛选出15株嗜热菌,并且对产中性蛋白酶活力进行测定，得到菌株FBKL1.016的中性蛋白酶活力最高，达到68.3 U/g，对其个体进行形态特征、生理生化试验及分子生物学鉴定，确定其为地衣芽孢杆菌。为进一步探索酱香酒产品风格提供了研究基础。