病毒感染复数影响腺病毒感染T淋巴细胞效率的初步研究

摘要

Previous studies have found that increasing multiplicity of infection (MOI) can effectively improve the efficiency of adenoviruse serotype 5 (Ad5) infection in T cells;however,little is known about the underlying mechanism.Here,Jurkat T cells were selected as target cells,and the effects of different MOI on the viral binding and viral entry in the replication cycle of Ad5-GFP were investigated by means of quantitative fluorescence PCR and transmission electron microscopy.It was found that high MOI significantly increased the number of adenovirus into the T cell during the endocytosis process,but not the number of adenovirus adsorbed on T cells.These results indicate that increase of MOI is beneficial to the adenovirus into the T cells by endocytosis,improving the efficiency of adenovirus infection in T lymphocytes,and this provide a theoretical basis for further study of the adenovirus infection mechanism in T lymphocytes.%以往研究发现,增加感染复数(multiplicity of infection,MOI)可以有效提高5型腺病毒(Ad5)感染T细胞的效率,但其具体机制并未十分清楚,选取T淋巴瘤细胞为靶细胞,通过荧光定量PCR及透射电镜观察等方法探讨不同MOI对重组腺病毒Ad5-GFP复制周期中病毒结合及病毒进入两个环节的影响,发现高MOI条件下不会影响T细胞结合的腺病毒数量,却可显著增加经胞吞进入T细胞的腺病毒数量.这些结果表明,增加MOI有利于腺病毒经胞吞进入T细胞,从而可提高腺病毒感染效率.这为进一步研究腺病毒感染T淋巴细胞的机制提供了理论基础.