婴儿配方奶粉中叶黄素测定

摘要

A method for determination of lutein in infant formula milk powder by HPLC is reported.HPLC on a Carotenoid C30 column（250.0 mm×4.6mm,5μm） with methanol-MTBE gradient elution,UV-vis detector,wavelength at 445nm for determenation of lutein.Linear ranges of lutein was 0.05～2.0μg/mL,the linear equation was：y=379874.03X＋14289.56,relevant index R2=0.9990;the content of milk powder were 50～500μg/100g,the recovery rate were 92.8～103.5%,and relative standard deviation was 1.2～3.5,the detectabke value is 5μg/100g.This method is fast,simple and accurate.%建立高效液相色谱法测定婴儿配方奶粉中叶黄素的检测方法。采用色谱柱为Carotenoid C30色谱柱（250.0mm×4.6mm,5μm）,流动相为甲醇和甲基叔丁基醚,梯度洗脱,紫外检测器,检测波长445nm。结果表明,叶黄素浓度在0.05～2.0μg/mL之间呈线性关系,线性方程为：y=379874.03X＋14289.56,相关系数R2=0.9990;奶粉的含量为50～500μg/100g时,回收率在92.8～103.5%之间,RSD%在1.2～3.5%之间;检出限为5μg/100g。该方法操作快捷简便,结果准确。