肿瘤血管导向性干扰素IFN-α2a-NGR与5-氟尿嘧啶联合应用对A549细胞增殖的影响

摘要

Objective: To observe the effects of IFN-α2a-NGR, 5-fluorouracil(5-FU) and their combination on the proliferation and apoptosis of A549 cell line. Methods: IFN-α2a-NGR(500 U/mL) and 5-FU(1.0 μg/mL) were used. A549 cells were divided into control group, IFN-α2a-NGR treated group, 5-FU treated group, IFN-α2a-NGR+5-FU treated group. The inhibition on the proliferation of A549 cells respectively treated with IFN-α2a-NGR, 5-FU and their combination was detected by MTF, and their apoptosis rate was detected by FCM, cell morphology was observed by fluorescence microscropy. Results: The inhibition rate and apoptosis rate of IFN-α2a-NGR+5-FU treated cells significantly higher than those of IFN-α2a-NGR alone treated cells and 5-FU alone treated cells. Conclusion: IFN-α2a-NGR combined with 5-FU can synergistically induce the inhibition and apoptosis of A549 cell line.%目的:观察重组蛋白肿瘤血管导向性干扰素IFN-α2a-NGR与化疗药物5-氟尿嘧啶联合使用在体外对人非小细胞肺癌细胞株A549的作用,为IFN-α2a-NGR的临床实验设计提供实验依据.方法:不同浓度的肿瘤血管导向性干扰素IFN-α2a-NGR、5-氟尿嘧啶单独及联合作用于细胞株A549,通过MTT法检测单药及联合用药对细胞增殖的影响,经Annexin V-FITC细胞凋亡检测试剂盒染色、流式细胞术检测细胞凋亡情况,荧光显微镜观察药物作用的细胞形态.结果:IFN-α2a-NGR与5-氟尿嘧啶联合作用于A549细胞株时,其细胞增殖抑制率、细胞凋亡率均较单独作用时高,具有统计学显著性差异(P<0.05);荧光显微镜观察可见药物作用后细胞形态改变.结论:肿瘤血管导向性干扰素IFN-α2a-NGR与5-氟尿嘧啶联合使用具有协同抑制肿瘤细胞增殖的作用.