蛋白质组学技术及其在药物成瘾研究中的应用

摘要

概述了蛋白质组学技术中分离、鉴定和定量方法及其在致瘾性药物(如酒精、烟碱、吗啡、可卡因和安非他命)研究中的应用.蛋白质组学技术是继后基因组学技术的一大重要突破.随着蛋白质分离、鉴定及定量技术的进步,尤其是覆盖范围广、分离效率高的液相色谱技术和高灵敏度质谱技术的发展,使得蛋白质组学越来越广泛地应用于药物成瘾研究,将有助于了解药物依赖过程中蛋白质的变化及其参与的生物过程和相关通路,理解药物成瘾的分子机制.%This paper briefly outlined the separation,identification and quantification proteomics technology and its application in research of addictive drugs like alcohol,nicotine,morphine,cocaine and methamphetamine.Proteomics is one of the powerful tools in the post-genomic era.With the development of protein separation,identification and quantitative technology,especially the liquid chromatography with wide application range and good separation efficiency and the mass spectrometry with high sensitivity,proteomics has been applied to drug addiction research more extensively,and will promote the understand of protein changes during the drug dependence formation and its participation in the biological process and related pathways,which will help to highlight molecular mechanisms underlying addiction.