衍生化气相色谱法测定冠心病患者血清中游离脂肪酸的方法学研究

摘要

目的：建立同时测定冠心病患者血清中11种游离脂肪酸（FFA）含量的测定方法。方法采用N，O-双（三甲基硅烷基）三氟乙酰胺（BSTFA）对11种FFA进行硅烷化反应，并利用气相色谱质谱联用技术（GC/MS）同时测定冠心病患者血清冻干粉中11种FFA的含量。结果11种FFA色谱峰完全分离，峰形良好，血清中杂质对测定不产生干扰。在测定浓度范围内线性关系良好，11种FFA的回收率均＞75％，日内、日间精密度和重复性实验的相对标准偏差（RSD）均＜10％。结论该方法具有操作简单、灵敏度高、准确性好、线性范围宽等优点，可应用于临床实际血清样品中FFA的分析检测。%Objective To establish a method for determining the serum concentrations of 11 free fatty acids(FFA) in patients with coronary disease.Methods The serum concentrations of 11 FFA in patients with coronary disease were determined by gas chromatography-mass spectrometry (GC/MS ) combined with derivation reaction and N,O-bis (trimethylsilyl)trifluoroacetamide(BSTFA)as derivative reagent.Results Chromatographic peaks of 11 FFA were separated completely,and all peak shapes were good.The interference of serum impurities on the determination did not occur.The curves showed good linearity.The recoveries of 11 FFA were >75%,respectively.The relative standard deviations(RSD)of intra-day and inter-day were<10%,respectively.Conclusions The method is sensitive,accurate, easy to operate and time-saving.It provides referential basis to the determination of serum FFA in coronary disease.