目的：探讨科宝Palio 300型尿全自动生化分析仪（简称Palio 300）对于5种尿微量蛋白项目的检测性能。方法同时采用Palio 300和西门子BN－prospec特定蛋白仪（简称BN－prospec ）检测120例患者尿微量白蛋白（mAlb）、尿α1微球蛋白（α1－MG）、尿β2微球蛋白（β2－MG）、尿转铁蛋白（TRF）、尿免疫球蛋白G（IgG），比较各项测定结果。按照项目分别配置中值及高值混合尿液，充分混匀后每份样本在Palio 300上重复测定20次，计算这5个项目的变异系数（ CV）。结果 Palio 300上5个项目除β2－MG CV为8．66％外，其余4项CV 均＜6．00％；且与BN－prospec的检测结果一致性较好，5个项目的r均＞0．9（其中mAlb为0．953、α1－MG为0．915、β2－MG为0．927、TRF为0．918、IgG为0．935， P均＜0．001）。结论 Palio 300具有较好的重复性，在测定5种尿mAlb项目时，与BN－prospec相关性良好。%Objective To evaluate the performance of Cobio Palio 300 urine automatic biochemical analyzer ( Palio 300) for the determinations of 5 kinds of urinary micro-proteins.Methods By Palio 300 and Siemens BN-prospec, 120 urine samples were determined for urinary micro-albumin ( mAlb ) , α1 microglobulin (α1-MG ) , β2 microglobulin (β2-MG) , transferrin ( TRF) and immunoglobulin G ( IgG) .The results were compared and analyzed statistically .In addition, the medium-value and high-value mixed urine was used for these items .Each sample was determined repeatedly for 20 times, and the coefficient of variation (CV) was calculated.Results Four items showed CV <6.00%, except β2-MG CV=8.66%.The results were consistent with those of Siemens BN-prospec.The correlation coefficients (r) of 5 items were >0.9 (mAlb 0.953,α1-MG 0.915,β2-MG 0.927, TRF 0.918 and IgG 0.935, P<0.001).Conclusions Palio 300 has good repeatability, and its result is consistent with Siemens BN-prospec in the determinations of 5 urinary micro-proteins .
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