Afinion AS100分析仪检测尿微量清蛋白、肌酐及其比值的准确性分析

摘要

Objective To discuss the accuracy of Afinion AS100 analyzer in detecting urinary microalbumin (MA),creatinine (Cre) and urinary albumin-creatinine ratio (ACR).Methods A total of 102 patients whose urinary protein qualitative results were(-)-(+) enrolled in the study,and fasting urinary MA,Cre and ACR were detected by Afinion AS100 analyzer with point-of-care testing (POCT).Meanwhile,urinary MA and Cre were measured by BN Ⅱ automatic protein analyzer and Hitachi 7600-110 automatic biochemical analyzer located in central laboratory respectively,and ACR were calculated.Then correlation of the two methods was compared.Results There were 17 cases without specific values of ACR detected by Afinion AS100 analyzer,and among which,15 cases showed ACR lower than 30 mg/g,which did not affect the judgment of the degree of proteinuria.For the other 85 cases,there were no significant differences in the values of MA,Cre and ACR between the two methods(P>0.05).Linear regression equations and correlation coefficients(r) of MA(Y=0.944X+3.879,r=0.982),Cre(Y=0.960X-0.188,r=0.963) and ACR(Y=1.196X+2.011,r=0.984) showed that two methods were positively correlated.Taken the test results of automatic analyzer as the evaluation standard,the probability of consistency of the AS100 Afinion analyzer to the degree of proteinuria was 92.2%.Conclusion Afinion AS100 analyzer can accurately determine the urinary MA,Cre and ACR of which urinary protein qualitative results are(-)-(+),and it can also be used to assess the degree of proteinuria.%目的 探讨Afinion AS100分析仪测定尿微量清蛋白(MA)、肌酐(Cre)和MA/Cre比值(ACR)的准确性.方法 应用Afinion AS100分析仪即时检测法测定102例尿蛋白定性(-)~(+)的患者清晨空腹尿MA、Cre和ACR,同步应用中心实验室BNⅡ全自动蛋白分析仪测定尿MA和Hitachi 7600-110全自动生化分析仪测定尿Cre,计算ACR值.比较两种方法的相关性.结果 Afinion AS100分析仪测定结果有17例ACR无具体数值,其中15例ACR<30 mg/g,不影响蛋白尿程度判断.其余85例,两种方法测定尿MA、Cre和ACR的数值差异均无统计学意义(P>0.05).MA、Cre和ACR的线性回归方程和相关系数(r)分别为:Y=0.944X+3.879,r=0.982;Y=0.960X-0.188,r=0.963;Y=1.196X+2.011,r=0.984.以BNⅡ和Hitachi 7600-110全自动分析仪测试结果为评估标准,Afinion AS100分析仪对蛋白尿程度评估一致性的概率为92.2%.结论 Afinion AS100分析仪可准确测定尿蛋白定性(-)~(+)患者的尿MA、Cre和ACR,评估蛋白尿程度.